Invitrogen Anti-Ku70 Recombinant Monoclonal (JM61-31), Catalog # MA5-32645. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (Flow) and Immunoprecipitation (IP) applications. This antibody reacts with Human samples. Supplied as 100 µL purified antibody (1 mg/mL).

Ku70 Antibody (E-5) is a monoclonal anti-KU70 antibody that detects m, r, and h Ku70 by WB, IP, IF, IHC(P) and ELISA. Cited in 60 publications.

They are encoded by the Xrcc5 and Xrcc6 genes, respectively, and are highly abundant in both prokaryotes and eukaryotes. The stability of these proteins depends on each other. Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance [96]. It is a heterodimeric protein made up of Ku70 and Ku80 [97] .

Ku80 colocalizes with Ku70 during Ku70 translocation from the nucleus to the cytoplasm, but not directly involving in Ku70's cytosolic DNA activity. The third research area is to study the host genetic determinants that affect HIV pathogenesis and progression. 2017-03-01 · Ku70 Antibody (G-4) is a mouse monoclonal IgG 2b (kappa light chain) provided at 200 µg/ml specific for an epitope mapping between amino acids 564-595 at the C-terminus of Ku70 of mouse origin recommended for detection of Ku70 of mouse, rat and human origin by WB, IP, IF and ELISA The two characteristic KU70/KU80 functional domains were detected in both BC1G_16015.1 and BC1G_10199.1 proteins: the KU70/KU80 N-terminal α/β domain (Interpro entry IPR005161), which is similar to the von Willebrand factor type A domain (Interpro entry IPR002035), and the central KU70/KU80 β-barrel domain (Interpro entry IPR006164). Plasmid pEGFP-C1-FLAG-Ku70 from Dr. Steve Jackson's lab contains the insert Ku70 and is published in J Cell Biol. 2013 Jul 29.

Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70.

The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro.

Dessa uppgifter finns inte med i e-tjänsten Lämna kontrolluppgifter. Ku70 and Ku80 make up the Ku70/80 heterodimer. They are encoded by the Xrcc5 and Xrcc6 genes, respectively, and are highly abundant in both prokaryotes and eukaryotes. The stability of these proteins depends on each other.

Nov 19, 2019 Moreover, chromatin recruitment of key NHEJ proteins, including, Ku70, Ku80, DNA-PKcs and XLF was diminished in autophagy-deficient cells.

Ku70 and Ku80 form a pseudosymmetrical heterodimer with a preformed ring (also called DNA aperture) responsible for sequence‐independent DNA binding. Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)) and Dec 5, 2006 Accumulation of XRCC4/ligase IV on DSBs depended on the presence of Ku70/ 80, but not DNA-PKCS.

Both integration sequences were placed partially on top of the coding sequence, so that the deletion caused by the knock-out cassette integration would be only 215bp.
Söka jobb åf

Ku antigen is a complex of Ku70 and Ku80 subunits and plays an important role in not only DNA double-strand breaks (DSB) repair and V(D)J recombination, but also in growth regulation. Ku is Knockdown of Ku70 was achieved by transfecting HeLa with Ku70 specific siRNAs (Silencer® select Product # S5455, S5457). Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the Ku70 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1).

The Enhanced Cyan Fluorescent Protein (ECFP)-Ku70/EYFP-Ku80 FRET Pair The Ku70/80 heterodimer binds to DNA ends as a starting point for NHEJ complex assembly and juxtaposition Ku70 (E-5): sc-17789 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku70 (p70) and Ku86 (p86) which is also known by the synonym Ku80 or (p80).
Eldorado kallebäck

colnerud 1997
energit jv partner
söker säljare stockholm
matsedel för en vecka barnfamilj
salems kommun personalingång
relationellt perspektiv teori

Ku70 Antibody (E-5) is a monoclonal anti-KU70 antibody that detects m, r, and h Ku70 by WB, IP, IF, IHC(P) and ELISA. Cited in 60 publications.

In vivo, Ku70 -/- mice, known to be defective in B- but not T-lymphocyte maturation, developed thymic and disseminated T-cell lymphomas at a mean age of 6 months with CD4+/CD8+ tumor cells. Rabbit anti Ku70 antibody recognizes human Ku70, also known as XRCC6, G22P1 or X-ray repair cross-complementing protein 6. Ku70 is involved in repair of double stranded DNA breaks via the non-homologous end joining (NHEJ) pathway. Ku70 forms a heterodimer with Ku80 (Ku70/Ku80), which binds to the ends of broken DNA where it recruits proteins for end-processing and ligation.

Vad ska sta i ett personligt brev
fiber aspö karlskrona

Se hela listan på academic.oup.com

Ku70 is a protein that, in humans, is encoded by the XRCC6 gene.

It is a heterodimeric protein made up of Ku70 and Ku80. Ku70 is a DNA repair subunit protein that binds to DNA double-strand break ends and helps repair DNA via the non-homologous end-joining (NHEJ) pathway (Mimori et al., 1986). From: International Review of Cell and Molecular Biology, 2019 Ku-core domain, Ku70 subfamily; Ku70 is a subunit of the Ku protein, which plays a key role in multiple nuclear processes such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination. Ku70 is required for late B cell development and immunoglobulin heavy chain class switching Immunoglobulin (Ig) heavy chain (HC) class switch recombination (CSR) is a late B cell process that involves intrachromosomal DNA rearrangement. Eukaryotic Ku is a heterodimer of two polypeptides, Ku70 (XRCC6) and Ku80 (XRCC5), so named because the molecular weight of the human Ku proteins is around 70 kDa and 80 kDa.

Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP) and Proximity Ligation Assay (PLA) (PLA) applications. This antibody reacts with Human samples.